Foodborne illness caused by spp. is one of the most prevalent public health problems globally, which have brought immeasurable economic burden and social impact to countries around the world. Neither current nucleic acid amplification detection method nor standard culture method (2–3 days) are suitable for field detection in areas with a heavy burden of spp. Here, we developed a highly sensitive and accurate assay for spp. detection in less than 40 min. Specifically, the gene of spp. was amplified by recombinase polymerase amplification (RPA), followed by Argonaute (Ago)-based target sequence cleavage, which could be observed by a fluorescence reader or the naked eye. The assay offered the lowest detectable concentration of 1.05 × 10 colony forming units/mL (CFU/mL). This assay had strong specificity and high sensitivity for the detection of spp. in field samples, which indicated the feasibility of this assay.

中文翻译：

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重组酶聚合酶扩增与激烈热球菌 Argonaute 相结合，可快速检测沙门氏菌。食品安全测试

由 spp. 引起的食源性疾病。是全球最普遍的公共卫生问题之一，给世界各国带来了难以估量的经济负担和社会影响。目前的核酸扩增检测方法和标准培养方法（2-3天）都不适合在菌群负担较重的地区进行现场检测。在这里，我们开发了一种高度灵敏且准确的检测方法。不到 40 分钟即可检测。具体来说，spp的基因。通过重组酶聚合酶扩增 (RPA) 进行扩增，然后进行基于 Argonaute (Ago) 的靶序列切割，可以通过荧光读数器或肉眼观察到。该测定提供的最低可检测浓度为 1.05 × 10 菌落形成单位/mL (CFU/mL)。该方法对 spp 的检测具有较强的特异性和较高的灵敏度。现场样品，表明该测定的可行性。