Tumor-marker immunosensors for rapid on-site detection have not yet been developed because of immunoreaction bottlenecks, such as shortening the reaction time and facilitating incubation. In this study, a gold–boron-nitrogen-codoped graphene (Au-BNG)-based immunosensor antenna was constructed for the rapid detection of neuron-specific enolase (NSE). A Au-BNG radiation electrode with dual functions of antibody protein fixation and signal transmission was developed for the first time. A radiation sample cell was constructed by embedding a radiation electrode into the groove of a poly(dimethylsiloxane) dielectric substrate. The constructed sense antenna achieves accurate detection of NSE with a range from 50 fg mL^–1 to 40,000 pg mL^–1 and a limit of detection of 10.99 fg mL^–1, demonstrating excellent selectivity, stability, and reliability. The tumor-marker detection meter can provide NSE detection results as rapidly as within 2 min by using the new strategy of the microwave self-incubation of tumor markers. This antenna immunosensor is suitable for rapid detection in outpatient clinics and can be developed into household tumor-marker detectors, which would be significant in the early detection, long-term monitoring, and efficacy evaluation of tumors.

中文翻译：

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用于快速检测神经元特异性烯醇化酶的无线金/硼氮共掺杂石墨烯天线免疫传感器

由于免疫反应瓶颈，例如缩短反应时间和促进孵育，用于快速现场检测的肿瘤标志物免疫传感器尚未开发出来。在这项研究中，构建了一种基于金硼氮共掺杂石墨烯（Au-BNG）的免疫传感器天线，用于快速检测神经元特异性烯醇化酶（NSE）。首次研制出具有抗体蛋白固定和信号传输双重功能的Au-BNG辐射电极。通过将辐射电极嵌入聚（二甲基硅氧烷）介电基板的凹槽中来构造辐射样品池。构建的传感天线实现了 NSE 的精确检测，范围为 50 fg mL ^–1至 40,000 pg mL ^–1，检测限为 10.99 fg mL ^–1，表现出优异的选择性、稳定性和可靠性。该肿瘤标志物检测仪采用肿瘤标志物微波自孵育新策略，可在2分钟内快速提供NSE检测结果。该天线免疫传感器适合门诊快速检测，可开发成家用肿瘤标志物检测仪，对肿瘤的早期检测、长期监测和疗效评估具有重要意义。