Three protein hydrolysates from were obtained by enzymatic hydrolysis employing two food-grade proteases ( Alcalase and Flavourzyme), and a complete characterisation of their composition was done. The digestion-derived products were obtained using the INFOGEST protocol. antioxidant activity and anti-inflammatory activities were evaluated. flour and the protein hydrolysates showed a high ability to scavenge the DPPH radical (EC values from 0.30 to 0.87 mg/mL). The hydrolysate obtained with a combination of the two food-grade proteases could decrease the gene expression of pro-inflammatory genes after being digested. Furthermore, the peptidome was fully determined for the first time for hydrolysates and digests, and 40 peptides were selected based on their bioactivity to be evaluated by tools, including prediction tools and molecular docking. These results provide new perspectives on the use of edible insects as sustainable and not nutritionally disadvantageous food for human consumption.

中文翻译：

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黄粉虫衍生寡肽的生产、表征和生物学特性

使用两种食品级蛋白酶（Alcalase 和 Flavourzyme）通过酶水解获得三种蛋白质水解产物，并对其组成进行了完整表征。使用 INFOGEST 方案获得消化衍生产物。评估了抗氧化活性和抗炎活性。面粉和蛋白质水解产物表现出很强的清除 DPPH 自由基的能力（EC 值从 0.30 到 0.87 mg/mL）。两种食品级蛋白酶组合获得的水解产物在消化后可以降低促炎基因的基因表达。此外，首次对水解产物和消化物的肽组进行了全面测定，并根据其生物活性筛选出40种肽，通过包括预测工具和分子对接在内的工具进行评估。这些结果为使用可食用昆虫作为人类消费的可持续且无营养不利的食物提供了新的视角。