Hydrophilic interaction liquid chromatography (HILIC) has always been devoted to the analysis of small polar compounds, glycans and glycopeptides. However, in the last 10 years HILIC has emerged as an analytical tool for the analysis of intact proteins, showing peculiar potentialities in the separation of protein and glycoprotein variants. The great compatibility with mass spectrometry detection and its unique complementarity with reverse phase chromatography makes HILIC an interesting option in intact protein characterization.

中文翻译：

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用于分析完整蛋白质和糖蛋白的亲水相互作用液相色谱 (HILIC)

亲水相互作用液相色谱（HILIC）一直致力于小极性化合物、聚糖和糖肽的分析。然而，在过去 10 年中，HILIC 已成为分析完整蛋白质的分析工具，在分离蛋白质和糖蛋白变体方面显示出独特的潜力。与质谱检测的良好兼容性及其与反相色谱的独特互补性使 HILIC 成为完整蛋白质表征的有趣选择。