SummaryA recent increase in the demand for pineapple necessitates the development of new varieties with superior flavour quality. In this study, we characterised the volatile profiles of three new Okinawan pineapple breeding lines using gas chromatography (GC)–mass spectrometry (MS)‐based electronic nose (e‐nose), glycosylated volatiles, and alcohol‐acyltransferase (AAT) activity measurements. The volatile profiles of the fruits were selected using the GC–MS‐e‐nose and visualised using multivariate statistical plots. The glycosidically bound compounds were collected by solid‐phase extraction and hydrolysed by β‐glucosidase. In total, 12 hydroxyl‐group substances, including chavicol (medicinal‐herbal aroma), geraniol (floral‐sweet), and linalool (floral‐citrus), were released from these glycosides. Crude enzymes containing AAT were extracted, and their in vitro activities in 2‐methylbutyl acetate production were highly related to their contents. These results provide information regarding volatile profiles and bounded‐aroma resources, as well as the esterification potential of new Okinawan pineapple breeding lines for use in the agro‐food industry.

中文翻译：

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基于气相色谱-质谱法的冲绳菠萝电子鼻、糖苷结合挥发物和醇酰基转移酶活性特征

摘要最近对菠萝的需求增加，需要开发具有卓越风味品质的新品种。在这项研究中，我们使用基于气相色谱 (GC) 和质谱 (MS) 的电子鼻 (e-nose)、糖基化挥发物和醇酰基转移酶 (AAT) 活性测量来表征三个新的冲绳菠萝育种品系的挥发物特征。使用 GC-MS-e-nose 选择水果的挥发性成分，并使用多元统计图进行可视化。通过固相萃取收集糖苷结合的化合物并通过水解β‐葡萄糖苷酶。这些糖苷总共释放出 12 种羟基物质，包括胡椒酚（药草香气）、香叶醇（花香甜味）和芳樟醇（花香柑橘香气）。提取含有AAT的粗酶，并对其体外乙酸2-甲基丁酯生产中的活性与其含量高度相关。这些结果提供了有关挥发性成分和有限香气资源的信息，以及用于农业食品工业的新冲绳菠萝育种品系的酯化潜力。