Among the primary threats to human health worldwide, nonsmall cell lung cancer (NSCLC) remains a significant factor and is a leading cause of cancer-related deaths. Due to subtle early symptoms, NSCLC patients are diagnosed at advanced stages, resulting in low survival rates. Herein, novel Au–Se bond nanoprobes (NPs) designed for the specific detection of Calpain-2 (CAPN2) and Human Neutrophil Elastase (HNE), pivotal biomarkers in NSCLC, were developed. The NPs demonstrated exceptional specificity and sensitivity toward CAPN2 and HNE, enabling dual-color fluorescence imaging to distinguish between NSCLC cells and normal lung cells effectively. The NPs’ performance was consistent across a wide pH range (6.2 to 8.0), and it exhibited remarkable resistance to biological thiol interference, indicating its robustness in complex physiological environments. These findings suggest the nanoprobe is a promising tool for early NSCLC diagnosis, offering a novel approach for enhancing the accuracy of cancer detection.

中文翻译：

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通过双重检测荧光纳米探针提高非小细胞肺癌诊断准确性

在全球人类健康的主要威胁中，非小细胞肺癌（NSCLC）仍然是一个重要因素，也是癌症相关死亡的主要原因。由于早期症状微妙，NSCLC患者确诊时已属晚期，导致生存率较低。在此，开发了新型金硒键纳米探针（NP），旨在特异性检测非小细胞肺癌（NSCLC）的关键生物标志物钙蛋白酶2（CAPN2）和人中性粒细胞弹性蛋白酶（HNE）。 NP 对 CAPN2 和 HNE 表现出卓越的特异性和敏感性，使双色荧光成像能够有效地区分 NSCLC 细胞和正常肺细胞。纳米粒子的性能在较宽的pH范围（6.2至8.0）内保持一致，并且表现出对生物硫醇干扰的显着抵抗力，表明其在复杂的生理环境中的稳健性。这些发现表明纳米探针是早期非小细胞肺癌诊断的一种有前途的工具，为提高癌症检测的准确性提供了一种新方法。