Plasma membrane intrinsic proteins () play important roles in plant growth, development, and abiotic stress responses. However, the functions and regulatory mechanisms of under high vapor pressure deficit (VPD) stress are not yet well understood. In this study, we used tobacco rattle virus (TRV) - mediated virus-induced gene silencing (VIGS) technology to discover that is involved in the acclimation process of tomato plants to high VPD. Under high VPD, the TRV- strain exhibited improved growth compared to the TRV strain, and silencing increased the enzyme activities of the antioxidant system of tomato plants and reduced the sensitivity of plants to atmospheric drought stress by eliminating reactive oxygen species produced by the plants. Additionally, leaf structure was improved, with decreased thickness of the epidermis and spongy tissue, leading to enhanced water transport efficiency. Furthermore, the TRV- strain had increased stomatal length and individual stomatal area, which changed net assimilation rate and stomatal conductance to air drought. In addition, was localized on the plasma membrane, and its promoter region contained numerous core elements and V-myb avian myeloblastosis viral oncogene homolog (MYB) binding sites. Yeast One-Hybrid Assay (Y1H) and Dual Luciferase (LUC) assays showed that the transcription factor SlMYB110 can bind to and regulate its expression. qRT-PCR analysis revealed that high VPD inhibited the expression of , consistent with the expression pattern of . Therefore, we speculate that under high VPD, SlMYB110 negatively regulates the expression of , thereby modulating the tolerance of tomato plants to atmospheric drought. These findings provide a theoretical basis for improving tomato response to high VPD, and was added to the high VPD-responsive gene pool as a valuable gene that could be used to improve high VPD tolerance in tomato through genetic engineering.

中文翻译：

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质膜内在蛋白 SlPIP2;5 基因调节番茄对高 VPD 的耐受性

质膜内在蛋白 () 在植物生长、发育和非生物胁迫反应中发挥重要作用。然而，高蒸气压赤字（VPD）压力下的功能和调节机制尚不清楚。在这项研究中，我们利用烟草脆裂病毒（TRV）介导的病毒诱导基因沉默（VIGS）技术来发现它参与了番茄植株对高VPD的适应过程。在高VPD下，TRV-菌株与TRV菌株相比表现出更好的生长，沉默增加了番茄植株抗氧化系统的酶活性，并通过消除植物产生的活性氧降低了植物对大气干旱胁迫的敏感性。此外，叶子结构得到改善，表皮和海绵组织的厚度减少，从而提高了水分输送效率。此外，TRV-菌株的气孔长度和单个气孔面积增加，这改变了对空气干旱的净同化率和气孔导度。此外，它位于质膜上，其启动子区域包含许多核心元件和V-myb禽成髓细胞瘤病毒癌基因同源物（MYB）结合位点。酵母单杂交实验（Y1H）和双荧光素酶（LUC）实验表明转录因子SlMYB110可以结合并调节其表达。 qRT-PCR 分析显示，高 VPD 抑制 的表达，与 的表达模式一致。因此，我们推测在高VPD下，SlMYB110负向调节 的表达，从而调节番茄植株对大气干旱的耐受性。这些研究结果为提高番茄对高VPD的反应提供了理论依据，并作为有价值的基因添加到高VPD反应基因库中，可用于通过基因工程提高番茄的高VPD耐受性。