The bioconversion of lignocellulosic biomass into novel bioproducts is crucial for sustainable biorefineries, providing an integrated solution for circular economy objectives. The current study investigated a novel microwave-assisted acidic deep eutectic solvent (DES) pretreatment of waste cocoa pod husk (CPH) biomass to extract xylooligosaccharides (XOS). The sequential DES (choline chloride/citric acid, molar ratio 1:1) and microwave (450W) pretreatment of CPH biomass was effective in 67.3% xylan removal with a 52% XOS yield from total xylan. Among different XOS of varying degrees of polymerization, a higher xylobiose content corresponding to 69.3% of the total XOS (68.22 mg/g CPH) from liquid fraction was observed. Enzymatic hydrolysis of residual xylan from pretreated CPH biomass with low commercial xylanase (10 IU/g) concentration yielded 24.2% XOS. The MW-ChCl/citric acid synergistic pretreatment approach holds great promise for developing a cost-effective and environmentally friendly method contributing to the sustainable production of XOS from agricultural waste streams.

中文翻译：

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微波和酸性低共熔溶剂基协同预处理可可豆荚壳生物质用于低聚木糖生产

将木质纤维素生物质生物转化为新型生物产品对于可持续生物精炼厂至关重要，为循环经济目标提供了综合解决方案。目前的研究研究了一种新型微波辅助酸性低共熔溶剂 (DES) 预处理废可可豆荚壳 (CPH) 生物质以提取低聚木糖 (XOS) 的方法。对 CPH 生物质进行连续 DES（氯化胆碱/柠檬酸，摩尔比 1:1）和微波 (450W) 预处理可有效去除 67.3% 的木聚糖，总木聚糖的 XOS 产率为 52%。在不同聚合度的不同 XOS 中，观察到较高的木二糖含量，相当于液体部分中总 XOS (68.22 mg/g CPH) 的 69.3%。使用低商业木聚糖酶 (10 IU/g) 浓度对预处理的 CPH 生物质中的残留木聚糖进行酶水解，产生 24.2% XOS。 MW-ChCl/柠檬酸协同预处理方法对于开发一种经济有效且环境友好的方法具有广阔的前景，有助于从农业废物流中可持续生产低聚木糖。