Hydrothermally prepared copper-doped carbon dots (Cu-CDs) were modified with Ca²⁺, which serve as an excellent platform for the recognition of glycine. The feeble emission of Ca@Cu-CD increases substantially in the presence of glycine due to aggregation-induced emission. At the same time, there was a 5-fold increase in the current response of the Ca@Cu-CD modified electrode as compared to the control. The exceptional combination of fluorescence and conducting properties, along with Ca-glycine interaction, establishes our probe as a dual sensor for the detection of glycine in real serum samples. The limit of detection for this nonenzymatic fluorescence and electrochemical sensing are 17.2 and 4.1 nM, respectively. Furthermore, an extensive evaluation of the toxicity and bioimaging properties in fruit fly Drosophila melanogaster shows that the Ca@Cu-CD probe is not cytotoxic and can be applied for ex vivo imaging of glycine.

中文翻译：

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Ca@Cu-CD 纳米探针用于甘氨酸双重检测和离体甘氨酸成像

水热制备的铜掺杂碳点（Cu-CDs）经过Ca ²⁺修饰，为甘氨酸的识别提供了良好的平台。由于聚集诱导的发射，Ca@Cu-CD 的微弱发射在甘氨酸存在下显着增加。同时，与对照相比，Ca@Cu-CD修饰电极的电流响应增加了5倍。荧光和导电特性的特殊组合，以及 Ca-甘氨酸相互作用，使我们的探针成为检测真实血清样品中甘氨酸的双传感器。这种非酶荧光和电化学传感的检测限分别为 17.2 和 4.1 nM。此外，对果蝇果蝇毒性和生物成像特性的广泛评估表明，Ca@Cu-CD探针不具有细胞毒性，可用于甘氨酸的离体成像。