Brassinosteroids (BRs) are plant steroidal hormones that play crucial roles in plant growth and development. Accurate quantification of BRs in plant tissues is essential for understanding their biological functions. This study presents a comprehensive overview of the latest methods used for the quantification of BRs in plants. We discuss the principles, advantages, and limitations of various analytical techniques, including immunoassays, gas chromatography-mass spectrometry (GC-MS), and liquid chromatography-tandem mass spectrometry (LC-MS/MS) that are used for the detection and quantification of BRs from complex plant matrices. We also explore the use of isotopically labeled internal standards to improve the accuracy and reliability of BR quantification.

中文翻译：

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油菜素类固醇分析的分析方法：最新进展和应用

油菜素内酯（BR）是植物甾体激素，在植物生长和发育中发挥着至关重要的作用。植物组织中 BR 的准确定量对于了解其生物学功能至关重要。本研究全面概述了用于植物中 BR 定量的最新方法。我们讨论了用于检测和定量的各种分析技术的原理、优点和局限性，包括免疫分析、气相色谱-质谱 (GC-MS) 和液相色谱-串联质谱 (LC-MS/MS)来自复杂植物基质的 BR。我们还探索使用同位素标记的内标来提高 BR 定量的准确性和可靠性。