BackgroundThe RNA‐binding motif single‐stranded interacting protein 3 (RBMS3) is a constituent of the RNA‐binding motif (RBM) protein family, which assumes a pivotal role in governing cellular biogenesis processes such as the cell cycle and apoptosis. Despite an abundance of studies elucidating RBMS3's divergent roles in the genesis and advancement of various tumors, its involvement in colon cancer remains enigmatic.MethodsThe present investigation employed data analysis from TCGA and GTEx to unveil that RBMS3 expression demonstrated a diminished presence in colon cancer tissues when juxtaposed with normal colon tissues. The effect of RBMS3 and LIM zinc finger domain 1 (LIMS1) on colon cancer was substantiated via animal models and cellular experiments. The connection between RBMS3 and LIM zinc finger domain 1 (LIMS1) was verified by molecular biology methods.ResultsThe study conclusively ascertained that augmenting RBMS3 expression quells the proliferation, migration, and invasion of colon cancer cells. Furthermore, the inquiry unveiled a plausible mechanism through which RBMS3 impacts the expression of LIMS1 by modulating its mRNA stability. The investigation ascertained that RBMS3 inhibits the progression of colon cancer by regulating LIMS1. The inhibitory function of LIMS1 and RBMS3 is closely intertwined in colon cancer, with knocking down LIMS1 being able to rescue the inhibitory effect of RBMS3 overexpression on the functionality of colon cancer cellConclusionsThe discernments delineate RBMS3 as a novel suppressor of cancer via LIMS1, thereby bestowing fresh therapeutic possibilities and illuminating the intricacies of colon cancer.

中文翻译：

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RNA结合蛋白RBMS3通过调节LIMS1 mRNA的稳定性抑制结肠癌的进展

背景RNA结合基序单链相互作用蛋白3（RBMS3）是RNA结合基序（RBM）蛋白家族的一个组成部分，在控制细胞周期和细胞凋亡等细胞生物发生过程中发挥着关键作用。尽管大量研究阐明了 RBMS3 在各种肿瘤的发生和进展中的不同作用，但它与结肠癌的关系仍然是个谜。 方法本研究采用 TCGA 和 GTEx 的数据分析来揭示，当与正常结肠组织并列。 RBMS3 和 LIM 锌指结构域 1 (LIMS1) 对结肠癌的作用已通过动物模型和细胞实验得到证实。通过分子生物学方法验证了 RBMS3 和 LIM 锌指结构域 1 (LIMS1) 之间的联系。结果该研究最终确定，增加 RBMS3 表达可抑制结肠癌细胞的增殖、迁移和侵袭。此外，该研究揭示了一种合理的机制，RBMS3 通过调节 LIMS1 的 mRNA 稳定性来影响 LIMS1 的表达。研究确定RBMS3通过调节LIMS1抑制结肠癌的进展。 LIMS1 和 RBMS3 的抑制功能在结肠癌中紧密相连，敲低 LIMS1 能够挽救 RBMS3 过表达对结肠癌细胞功能的抑制作用。治疗的可能性并阐明结肠癌的复杂性。