As people age, their ability to resist injury and repair damage decreases significantly. Platelet-rich plasma (PRP) has demonstrated diverse therapeutic effects on tissue repair. However, the inconsistency of patient outcomes poses a challenge to the practical application of PRP in clinical practice. Furthermore, a comprehensive understanding of the specific impact of aging on PRP requires a systematic investigation. We derived PRP from 6 young volunteers and 6 elderly volunteers, respectively. Subsequently, 95% of high-abundance proteins were removed, followed by mass spectrometry analysis. Data are available via ProteomeXchange with the identifier PXD050061. We detected a total of 739 proteins and selected 311 proteins that showed significant differences, including 76 upregulated proteins in the young group and 235 upregulated proteins in the elderly group. Functional annotation and enrichment analysis unveiled upregulation of proteins associated with cell apoptosis, angiogenesis, and complement and coagulation cascades in the elderly. Conversely, IGF1 was found to be upregulated in the young group, potentially serving as the central source of enhanced cell proliferation ability. Our investigation not only provides insights into standardizing PRP preparation but also offers novel strategies for augmenting the functionality of aging cells or tissues.

中文翻译：

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年轻人和老年人富含血小板的血浆表现出不同的蛋白质组特征

随着人们年龄的增长，抵抗伤害和修复损伤的能力显着下降。富含血小板血浆（PRP）已证明对组织修复有多种治疗作用。然而，患者结果的不一致给PRP在临床实践中的实际应用带来了挑战。此外，全面了解衰老对 PRP 的具体影响需要进行系统的研究。我们分别从 6 名年轻志愿者和 6 名老年志愿者中获得了 PRP。随后，去除 95% 的高丰度蛋白质，然后进行质谱分析。数据可通过 ProteomeXchange 获得，标识符为 PXD050061。我们总共检测了739个蛋白质，筛选出311个表现出显着差异的蛋白质，其中年轻组中上调的蛋白质有76个，老年组中上调的蛋白质有235个。功能注释和富集分析揭示了与老年人细胞凋亡、血管生成、补体和凝血级联相关的蛋白质的上调。相反，IGF1被发现在年轻组中表达上调，可能是细胞增殖能力增强的主要来源。我们的研究不仅提供了标准化 PRP 制备的见解，还提供了增强衰老细胞或组织功能的新策略。