The development of intelligent, sensitive, and visual methods for the rapid detection of veterinary drug residues is essential to ensure food quality and safety. Here, a smartphone-based dual inverse signal MOFs fluorescence sensing system was proposed for intelligent in-site visual detection of malachite green (MG). A UiO-66-NH@RhB-dual-emission fluorescent probe was successfully synthesized in one step using a simple one-pot method. The inner filter effect (IFE) quenches the red fluorescence, while hydrogen bonding interaction enhances the blue fluorescence, enabling highly sensitive, accurate, and visual detection of MG dual inverse signals through fluorescence analysis. The probe showed great linearity over a wide range of 0.1–100 μmol/L, with a limit of detection (LOD) of 20 nmol/L. By integrating smartphone photography and RGB (red, green, and blue) analysis, accurate quantitative analysis of MG in water and actual fish samples can be achieved within 5 min. This developed platform holds great promise for the on-site detection of MG in practical applications, with the advantages of simplicity, cost-effectiveness, and rapidity. Consequently, it may open up a new pathway for on-site evaluation of food safety and environmental health.

中文翻译：

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基于智能手机的双反信号 MOF 荧光传感，用于孔雀石绿的智能现场视觉检测

开发智能、灵敏、可视化的兽药残留快速检测方法对于确保食品质量和安全至关重要。这里，提出了一种基于智能手机的双反信号MOF荧光传感系统，用于孔雀石绿（MG）的智能现场视觉检测。采用简单的一锅法一步成功合成了 UiO-66-NH@RhB 双发射荧光探针。内滤光效应 (IFE) 淬灭红色荧光，而氢键相互作用增强蓝色荧光，从而通过荧光分析实现 MG 双反信号的高灵敏、准确和可视化检测。该探针在 0.1–100 μmol/L 的宽范围内表现出良好的线性，检测限 (LOD) 为 20 nmol/L。通过集成智能手机摄影和RGB（红、绿、蓝）分析，可以在5分钟内实现水体和实际鱼类样本中MG的精确定量分析。该平台具有简单、经济、快速等优点，为实际应用中的MG现场检测带来了广阔的前景。因此，它可能为食品安全和环境健康的现场评价开辟一条新途径。