Sunset Yellow (SY), an azo synthetic food dye, is widely used in the food industry. Although there are different opinions on its effect on people, its use is regulated in the European Union. If the Acceptable Daily Intake of 2.5 mg/kg/bw is exceeded, it may have pathological and biochemical effects on organs. There are not enough studies on the effects of SY on growth and development in mammals. This study was conducted to determine the effect of SY on the morphological parameters of mice at different ages (four, eight, and ten weeks old). The treatment and control groups were created with Swiss Albino mice (n: 6). SY was administered orally for 28 days (30 mg/kg/bw/week). On the last day of the study, the mice were weighed, and tail, temporal region, femur, and crown-rubmp-length values were measured using a digital caliper. A statistical difference in average body weight was observed in the SY groups (p < 0.05). SY administration during childhood caused retardation in growth and development parameters. Therefore, SY may cause weight gain and affect morphological parameters. Additional studies are required to investigate the effects of SY at different doses and durations.

中文翻译：

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日落黄对雄性小鼠发育期形态参数影响的测定

日落黄（SY）是一种偶氮合成食用染料，广泛应用于食品工业。尽管对其对人的影响存在不同意见，但其使用在欧盟受到监管。如果超过每日可接受摄入量2.5毫克/公斤/体重，可能会对器官产生病理和生化影响。关于SY对哺乳动物生长发育的影响还没有足够的研究。本研究旨在确定 SY 对不同年龄（4、8 和 10 周龄）小鼠形态参数的影响。治疗组和对照组是用瑞士白化小鼠（n：6）创建的。 SY 口服给药 28 天（30 毫克/公斤/体重/周）。在研究的最后一天，对小鼠进行称重，并使用数字卡尺测量尾部、颞区、股骨和冠脉长度值。在 SY 组中观察到平均体重存在统计学差异 (p < 0.05)。儿童时期服用 SY 会导致生长和发育参数迟缓。因此，SY可能导致体重增加并影响形态参数。需要进行更多研究来调查 SY 在不同剂量和持续时间下的影响。