Larvae of the nematode are capable of causing parasitic infections in humans after the consumption of uncooked meat or meat products, leading to severe symptoms and even death. Meat inspection is costly and tends to progressively be derogated in holdings applying controlled housing conditions in certain regions. Our study focuses on the evaluation of the efficacy of Pulsed Electric Fields (PEF) in the inactivation of spp., including excysted larvae isolated by artificial digestion and encapsulated larvae found in meat from naturally infected wild boars. Microscopic examination of unstained and propidium-iodide-stained larvae suspensions showed that most of the excysted larvae were inactivated 10 min after an intermediate PEF treatment (1 kV/cm, 0.41 kJ/kg). Treating excysted larvae with the mildest PEF treatment (0.5 kV/cm, 0.05 kJ/kg) combined with a 3% NaCl incubation resulted in synergistic inactivation. The application of 3 kV/cm (20 kJ/kg) to wild boar meat resulted in the inactivation of over 90% of encapsulated larvae. The viability of in meat was inversely correlated to the field strength applied (1–6 kV/cm) for equal energy input (20 kJ/kg). These findings demonstrate that PEF technology can potentially serve as a novel strategy for the inactivation of larvae in meat.

中文翻译：

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旋毛虫属的灭活。脉冲电场 (PEF) 处理后自然感染的猪肉中

食用未煮熟的肉或肉制品后，线虫幼虫能够在人体中引起寄生虫感染，导致严重症状甚至死亡。肉类检验成本高昂，而且在某些地区实行受控饲养条件的农场中，肉类检验往往会逐渐受到削弱。我们的研究重点是评估脉冲电场 (PEF) 在灭活某些物种方面的功效，包括通过人工消化分离的包囊幼虫和在自然感染野猪的肉中发现的包囊幼虫。对未染色和碘化丙啶染色的幼虫悬浮液进行显微镜检查表明，大多数排出的幼虫在中间 PEF 处理（1 kV/cm，0.41 kJ/kg）后 10 分钟被灭活。用最温和的 PEF 处理（0.5 kV/cm，0.05 kJ/kg）结合 3% NaCl 孵育来处理囊肿幼虫，导致协同灭活。对野猪肉施加 3 kV/cm (20 kJ/kg) 的电压导致超过 90% 的包囊幼虫失活。对于相同的能量输入（20 kJ/kg），肉中的活力与所施加的场强（1-6 kV/cm）成反比。这些发现表明，PEF 技术有可能成为一种灭活肉类中幼虫的新策略。