The rete ovarii (RO) is an epithelial structure that arises during development in close proximity to the ovary and persists throughout adulthood. However, the functional significance of the RO remains elusive, and it is absent from recent discussions of female reproductive anatomy. The RO comprises three regions: the intraovarian rete within the ovary, the extraovarian rete in the periovarian tissue, and the connecting rete linking the two. We hypothesize that the RO plays a pivotal role in ovarian homeostasis and responses to physiological changes. To begin to uncover the nature and function of RO cells, we conducted transcriptomic profiling of the RO. This study presents three datasets, and reports our analysis and quality control approaches for bulk, single-cell, and nucleus-level transcriptomics of the fetal and adult RO tissues using the Pax8-rtTA; Tre-H2B-GFP mouse line, where all RO regions express nuclear GFP. The integration and rigorous validation of these datasets will advance our understanding of the RO’s roles in ovarian development, female maturation, and adult female fertility.

卵巢网 (RO) 是一种上皮结构，在发育过程中出现，靠近卵巢，并在整个成年期持续存在。然而，RO 的功能意义仍然难以捉摸，并且在最近的女性生殖解剖学讨论中也没有出现。 RO 由三个区域组成：卵巢内的卵巢内网、卵巢周围组织中的卵巢外网以及连接两者的连接网。我们假设 RO 在卵巢稳态和对生理变化的反应中发挥着关键作用。为了开始揭示 RO 细胞的性质和功能，我们对 RO 进行了转录组分析。这项研究提供了三个数据集，并报告了我们使用Pax8-rtTA对胎儿和成人 RO 组织进行批量、单细胞和细胞核水平转录组学的分析和质量控制方法； Tre-H2B-GFP小鼠系，其中所有 RO 区域均表达核 GFP。这些数据集的整合和严格验证将增进我们对 RO 在卵巢发育、女性成熟和成年女性生育力中的作用的理解。