Wide‐field imaging is widely adopted due to its fast acquisition, cost‐effectiveness, and ease of use. Its extension to direct volumetric applications, however, is burdened by the trade‐off between resolution and depth of field (DOF), dictated by the numerical aperture of the system. It is demonstrated that such trade‐off is not intrinsic to wide‐field imaging, but stems from the spatial incoherence of light: images obtained through spatially coherent illumination are shown to have resolution and DOF independent of the numerical aperture. This fundamental discovery enables to demonstrate an optimal combination of coherent resolution‐DOF enhancement and incoherent tomographic sectioning for scanning‐free, wide‐field 3D microscopy on a multicolor histological section.

中文翻译：

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通过光的空间相干性进行直接 3D 成像

宽视场成像因其快速采集、成本效益和易用性而被广泛采用。然而，它扩展到直接体积应用，受到分辨率和景深 (DOF) 之间的权衡的影响，而景深 (DOF) 由系统的数值孔径决定。事实证明，这种权衡并不是广域成像所固有的，而是源于光的空间不相干性：通过空间相干照明获得的图像显示出具有与数值孔径无关的分辨率和自由度。这一基本发现能够证明相干分辨率 DOF 增强和不相干断层扫描切片的最佳组合，用于多色组织切片上的免扫描、宽视场 3D 显微镜。