SummaryThe nuclear factor Y (NF‐Y) transcription factors play important roles in plant development and physiological responses. However, the relationship between NF‐Y, plant hormone and plant stress resistance in tropical crops remains unclear. In this study, we identified MeNF‐YC15 gene in the NF‐Y family that significantly responded to Xanthomonas axonopodis pv. manihotis (Xam) treatment. Using MeNF‐YC15‐silenced and ‐overexpressed cassava plants, we elucidated that MeNF‐YC15 positively regulated disease resistance to cassava bacterial blight (CBB). Notably, we illustrated MeNF‐YC15 downstream genes and revealed the direct genetic relationship between MeNF‐YC15 and 1‐aminocyclopropane‐1‐carboxylic acid (ACC) oxidase (MeACO1)‐ethylene module in disease resistance, as evidenced by the rescued disease susceptibility of MeNF‐YC15 silenced cassava plants with ethylene treatment or overexpressing MeACO1. In addition, the physical interaction between 2C‐type protein phosphatase 1 (MePP2C1) and MeNF‐YC15 inhibited the transcriptional activation of MeACO1 by MeNF‐YC15. In summary, MePP2C1‐MeNF‐YC15 interaction modulates ethylene biosynthesis and cassava disease resistance, providing gene network for cassava genetic improvement.

中文翻译：

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NF-YC15转录因子激活乙烯生物合成并提高木薯抗病性

摘要核因子 Y (NF-Y) 转录因子在植物发育和生理反应中发挥着重要作用。然而，NF-Y、植物激素和热带作物植物抗逆性之间的关系仍不清楚。在这项研究中，我们确定了MeNF-YC15NF-Y 家族中的基因对轴叶黄单胞菌光伏。木薯属（西姆） 治疗。使用MeNF-YC15‐沉默和过度表达木薯植物，我们阐明 MeNF-YC15 正向调节木薯白叶枯病 (CBB) 的抗病性。值得注意的是，我们阐明了 MeNF-YC15 下游基因，并揭示了 MeNF-YC15 与 1-氨基环丙烷-1-羧酸 (ACC) 氧化酶 (MeACO1)-乙烯模块在抗病性方面的直接遗传关系，这一点可以通过挽救的疾病易感性来证明。MeNF-YC15通过乙烯处理或过度表达沉默木薯植物MeACO1.此外，2C型蛋白磷酸酶1（MePP2C1）和MeNF-YC15之间的物理相互作用抑制了转录激活甲基ACO1通过 MeNF-YC15。总之，MePP2C1-MeNF-YC15 相互作用调节乙烯生物合成和木薯抗病性，为木薯遗传改良提供基因网络。