The perception of polyphenols as a safe, healthy, and sustainable solution for replacing synthetic antioxidants has been an important factor for their rapid growing in the global food market. Therefore, it is essential to use reliable methods for their quantification in commercial products intended for animal or human consumption. The purpose of this study is to evaluate the performance of some solvents used for the extraction of selected polyphenols, explore their stability under different experimental conditions, and validate a liquid chromatography tandem mass-spectrometry method for their quantification in commercial fish feed ingredients by using the standard addition method. The regression models for gallic acid, hydroxytyrosol, catechin, oleuropein, carnosol and carnosic acid were linear in the range 0–30 μg/mL, limit of detection and quantification around 0.03 and 0.1 μg/mL, respectively, and accuracy within ± 15 % of the nominal concentrations. The method was successfully applied to the determination of specific polyphenols in commercial fish feed ingredients supplemented with polyphenols from olive and rosemary extracts.

中文翻译：

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液相色谱串联质谱法定量分析鱼饲料成分中多酚的稳定性研究和验证

人们认为多酚是一种安全、健康和可持续的替代合成抗氧化剂的解决方案，这是其在全球食品市场快速增长的一个重要因素。因此，必须使用可靠的方法对供动物或人类消费的商业产品进行定量。本研究的目的是评估用于提取选定多酚的一些溶剂的性能，探索它们在不同实验条件下的稳定性，并验证液相色谱串联质谱法用于商业鱼饲料成分中的定量。标准添加法。没食子酸、羟基酪醇、儿茶素、橄榄苦苷、鼠尾草酚和鼠尾草酸的回归模型在 0–30 μg/mL 范围内呈线性，检测限和定量限分别约为 0.03 和 0.1 μg/mL，准确度在 ± 15 % 以内的名义浓度。该方法成功应用于补充了橄榄和迷迭香提取物多酚的商业鱼饲料成分中特定多酚的测定。