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Detection of trace components in Xiangdan injection of Dalbergia odorifera based on microextraction and back-extraction along with bar-form-diagram strategy
Journal of Chromatography A ( IF 4.1 ) Pub Date : 2024-03-27 , DOI: 10.1016/j.chroma.2024.464852 Yuan Chen , Yanqi Yang , Yingfeng Pan , Xiaoyi Liu , Hongxu Zhang , Yixia Pan , Jizhong Yan , Hui Zhang
Journal of Chromatography A ( IF 4.1 ) Pub Date : 2024-03-27 , DOI: 10.1016/j.chroma.2024.464852 Yuan Chen , Yanqi Yang , Yingfeng Pan , Xiaoyi Liu , Hongxu Zhang , Yixia Pan , Jizhong Yan , Hui Zhang
Xiangdan Injection are commonly used traditional Chinese medicine formulations for the clinical treatment of cardiovascular diseases. However, the trace components of in Xiangdan Injection pose a challenge for evaluating its quality due to the difficulty of detection. This study proposes a technology combining dispersive liquid-liquid microextraction and back-extraction (DLLME-BE) along with Bar-Form-Diagram (BFD) to address this issue. The proposed combination method involves vortex-mixing tetradecane, which has a lower density than water, with the sample solution to facilitate the transfer of the target components. Subsequently, a new vortex-assisted liquid-liquid extraction step is performed to enrich the components of in acetonitrile. The sample analysis was performed on HPLC-DAD, and a clear overview of the chemical composition was obtained by integrating spectral and chromatographic information using BFD. The combination of BFD and CRITIC-TOPSIS strategies was used to optimize the process parameters of DLLME-BE. The determined optimal sample pre-treatment process parameters were as follows: 200 μL extraction solvent, 60 s extraction time, 50 μL back-extraction solvent, and 90 s back-extraction time. Based on the above strategy, a total of 29 trace components, including trans-nerolidol, were detected in the Xiangdan Injection. This combination technology provides valuable guidance for the enrichment analysis of trace components in traditional Chinese medicines.
更新日期：2024-03-27
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