Main conclusion

S. plumbizincicola genetic transformation was optimized using a self-excision molecular-assisted transformation system by integrating the SpGRF4/SpGIF1 gene with XVE and Cre/loxP.

Abstract

Sedum plumbizincicola, despite being an excellent hyperaccumulator of cadmium and zinc with significant potential for soil pollution phytoremediation on farmland, has nonetheless trailed behind other major model plants in genetic transformation technology. In this study, different explants and SpGRF4–SpGIF1 genes were used to optimize the genetic transformation of S. plumbizincicola. We found that petiole and stem segments had higher genetic transformation efficiency than cluster buds. Overexpression of SpGRF4–SpGIF1 could significantly improve the genetic transformation efficiency and shorten the period of obtaining regenerated buds. However, molecular assistance with overexpression of SpGRF4–SpGIF1 leads to abnormal morphology, resulting in plant tissue enlargement and abnormal growth. Therefore, we combined SpGRF4–SpGIF1 with XVE and Cre/loxP to obtain DNA autocleavage transgenic plants induced by estradiol, thereby ensuring normal growth in transgenic plants. This study optimized the S. plumbizincicola genetic transformation system, improved the efficiency of genetic transformation, and established a self-excision molecular-assisted transformation system. This work also established the basis for studying S. plumbizincicola gene function, and for S. plumbizincicola breeding and germplasm innovation.

中文翻译：

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利用 SpGRF4–SpGIF1 基因和自切除 CRE/LoxP 系统改善水景景天遗传转化

主要结论

通过将SpGRF4/SpGIF1基因与 XVE 和 Cre/loxP整合，使用自切除分子辅助转化系统优化了S. plumbizincicola遗传转化。

抽象的

尽管铅景天是一种优良的镉和锌超富集植物，在农田土壤污染植物修复方面具有巨大潜力，但在遗传转化技术方面仍落后于其他主要模式植物。本研究利用不同的外植体和SpGRF4 – SpGIF1基因来优化S. plumbizincicola的遗传转化。我们发现叶柄和茎段比簇芽具有更高的遗传转化效率。过表达SpGRF4 - SpGIF1可以显着提高遗传转化效率，缩短获得再生芽的时间。然而， SpGRF4-SpGIF1过度表达的分子协助会导致形态异常，导致植物组织增大和异常生长。因此，我们将SpGRF4–SpGIF1与XVE和Cre/loxP结合，获得雌二醇诱导的DNA自裂解转基因植物，从而保证转基因植物的正常生长。本研究优化了S. plumbizincicola遗传转化体系，提高了遗传转化效率，建立了自切除分子辅助转化体系。该工作也为研究梅花基因功能、梅花选育和种质创新奠定了基础。