In this study, a two-stage culture strategy was refined to concurrently enhance the growth and lipid accumulation of . The results unveiled that, during the initial stage, the optimal conditions for biomass accumulation were achieved with 0.02 mg·L Cu concentration and red light. Under these conditions, biomass accumulation reached 0.628 g·L, marking a substantial 23.62 % increase compared to the control group. In the second stage, the optimal conditions for lipid accumulation were identified as 0.5 mg·L Cu concentration and red light, achieving 64.25 mg·g·d and marking a 128.38 % increase over the control. Furthermore, the fatty acid analysis results revealed an 18.85 % increase in the saturated fatty acid content, indicating enhanced combustion performance of microalgae cultivated under the dual stress of red light and 0.5 mg·L Cu. This study offers insights into the potential application of in biofuel production.

中文翻译：

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铜胁迫和光质量诱导改良栽培策略，以提高斜叶四足藻 FACHB-14 的生物量产量和脂质积累

在这项研究中，改进了两阶段培养策略，以同时增强 的生长和脂质积累。结果表明，初始阶段生物量积累的最佳条件为0.02 mg·L Cu浓度和红光。在此条件下，生物量积累达到0.628 g·L，较对照组大幅增加23.62%。第二阶段，脂质积累的最佳条件为0.5 mg·L Cu浓度和红光，达到64.25 mg·g·d，比对照增加128.38%。此外，脂肪酸分析结果显示，饱和脂肪酸含量增加了18.85%，表明红光和0.5 mg·L Cu双重胁迫下培养的微藻的燃烧性能增强。这项研究为生物燃料生产中的潜在应用提供了见解。