A non-radical proximity labelling platform — BAP-seq — is presented that uses subcellular-localized BS2 esterase to convert unreactive enol-based probes into highly reactive acid chlorides in situ to label nearby RNAs. When paired with click-handle-mediated enrichment and sequencing, this chemistry enables high-resolution spatial mapping of RNAs across subcellular compartments.

中文翻译：

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用于 RNA 邻近标记的掩蔽酰基氯

提出了一种非自由基邻近标记平台——BAP-seq，该平台使用亚细胞定位的 BS2 酯酶将非反应性烯醇基探针原位转化为高反应性酰基氯，以标记附近的 RNA。当与点击手柄介导的富集和测序配合使用时，这种化学反应能够实现亚细胞区室中 RNA 的高分辨率空间图谱。