Main conclusion

The three, by mutagenesis produced genes OsPi21, OsXa5, and OsBADH₂, generated novel lines exhibiting desired fragrance and improved resistance.

Abstract

Elite sterile lines are the basis for hybrid rice breeding, and rice quality and disease resistance become the focus of new sterile lines breeding. Since there are few sterile lines with fragrance and high resistance to blast and bacterial blight at the same time in hybrid rice production, we here integrated the simultaneous mutagenesis of three genes, OsPi21, OsXa5, and OsBADH₂, into Zhi 5012S, an elite thermo-sensitive genic male sterile (TGMS) variety, using the CRISPR/Cas9 system, thus eventually generated novel sterile lines would exhibit desired popcorn-like fragrance and improved resistance to blast and bacterial blight but without a loss in major agricultural traits such as yield. Collectively, this study develops valuable germplasm resources for the development of two-line hybrid rice with disease resistance, which provides a way to rapid generation of novel TGMS lines with elite traits.

中文翻译：

---

通过 CRISPR/Cas9 快速培育具有增强抗病性的香型温敏基因雄性不育稻

主要结论

这三个基因通过诱变产生了OsPi21、OsXa5和OsBADH ₂，产生了表现出所需香味和改善抗性的新品系。

抽象的

优良不育系是杂交水稻育种的基础，稻米品质和抗病性成为新不育系选育的重点。由于杂交水稻生产中同时具有香味且抗稻瘟病和白叶枯病的不育系很少，因此我们将OsPi21、OsXa5和OsBADH ₂三个基因同时诱变，整合到优良耐热性质Zhi 5012S中。 -使用CRISPR/Cas9系统的敏感基因雄性不育（TGMS）品种，最终产生的新型不育系将表现出所需的爆米花香味，并提高对稻瘟病和白叶枯病的抗性，但不会损失产量等主要农业性状。总的来说，本研究为抗病两系杂交水稻的开发开发了宝贵的种质资源，为快速产生具有优良性状的新型TGMS品系提供了途径。