Reaction-based fluorescent probes can react with the target through one-step reaction induced by the collision between the analytes and the probes, and generally display good performance on sensitivity and selectivity. However, these probes occasionally show poor sensitivity and selectivity in complex system due to interference from competitive substances, and fail to detect substances with low reactivity. Molecular fluorescent probe with multi-recognition sites is a novel designing strategy which solves these problems by offering more binding/reaction sites to highly increase the interaction chance of the probe and the target, thus achieving improved recognition efficiency, resulting in high sensitivity and selectivity. Its advent also effectively helps monitor the ever-changing biomolecules even those at low concentrations. Herein, we discussed the designing paradigm of molecular fluorescent probes with multi-recognition sites, and their recent progress applied in crucial biomolecules, such as catecholamines, ATP and biothiols, . Finally, current challenges and future prospects are discussed.

中文翻译：

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具有多识别位点的分子荧光探针的最新进展使灵敏和选择性分析成为可能

基于反应的荧光探针可以通过分析物与探针之间的碰撞诱导的一步反应与目标物发生反应，并且通常在灵敏度和选择性方面表现出良好的性能。然而，这些探针在复杂系统中有时会因竞争性物质的干扰而表现出较差的灵敏度和选择性，并且无法检测反应活性较低的物质。具有多识别位点的分子荧光探针是一种新颖的设计策略，它通过提供更多的结合/反应位点来大大增加探针与靶标的相互作用机会，从而提高识别效率，从而提高灵敏度和选择性，从而解决了这些问题。它的出现还有效地帮助监测不断变化的生物分子，甚至是低浓度的生物分子。在此，我们讨论了具有多识别位点的分子荧光探针的设计范式，及其在儿茶酚胺、ATP和生物硫醇等关键生物分子中应用的最新进展。最后，讨论了当前的挑战和未来的前景。