Athetis lepigone is an emerging highly polyphagous insect pest reported to cause crop damage in several European and Asian countries. However, our understanding of its genetic adaptation mechanisms has been limited due to lack of high-quality genetic resources. In this study, we present a chromosomal-level genome of A. lepigone, representing the first species in the genus of Athetis. We employed PacBio long-read sequencing and Hi-C technologies to generate 612.49 Mb genome assembly which contains 42.43% repeat sequences with a scaffold N50 of 20.9 Mb. The contigs were successfully clustered into 31 chromosomal-size scaffolds with 37% GC content. BUSCO assessment revealed a genome completeness of 97.4% with 96.3 identified as core Arthropoda single copy orthologs. Among the 17,322 genes that were predicted, 15,965 genes were functionally annotated, representing a coverage of 92.17%. Furthermore, we revealed 106 P450, 37 GST, 27 UGT, and 74 COE gene families in the genome of A. lepigone. This genome provides a significant and invaluable genomic resource for further research across the entire genus of Athetis.

斑蛾是一种新出现的高度杂食性害虫，据报道在几个欧洲和亚洲国家造成作物损害。然而，由于缺乏优质遗传资源，我们对其遗传适应机制的了解一直有限。在这项研究中，我们提出了A. lepigone的染色体水平基因组，代表了Athetis属的第一个物种。我们采用 PacBio 长读长测序和 Hi-C 技术生成 612.49 Mb 的基因组组装，其中包含 42.43% 的重复序列，支架 N50 为 20.9 Mb。重叠群成功聚集成 31 个染色体大小的支架，GC 含量为 37%。 BUSCO 评估显示基因组完整性为 97.4%，其中 96.3 个被确定为核心节肢动物单拷贝直向同源物。在预测的17,322个基因中，有15,965个基因被功能注释，覆盖率为92.17%。此外，我们还揭示了A.lepigone基因组中的106个P450、37个GST、27个UGT和74个COE基因家族。该基因组为整个Athetis属的进一步研究提供了重要且宝贵的基因组资源。