In this work, a highly sensitive lung cancer biomarkers detection probe was developed based on Ag and MXene co-functionalized magnetic microspheres. By using carboxyl magnetic microspheres as carrier, MXene was coated repeatedly by Poly (allylamine hydrochloride) (PAH) as interlayer adhesive, and silver particles grown on the surface of MXene in situ can efficiently improve the sensitivity of the probe. The detection of neuron specific enolase (NSE) is mainly through the formation of a specific complex between NSE antigen and antibody, and the release of antibody labeled with amino carbon quantum dots (CQDs) from the surface of Ag nanoparticles (AgNPs), so that the fluorescence is restored and "OFF-ON" is formed. The biosensor exhibits excellently wide linear range (0.0001–1500 ng/mL) and the limit of detection (LOD) is up to 0.03 pg/mL, which is superior to most tumor marker probes based on fluorescence mechanism. Furthermore, we constructed dual detection strategy for NSE and carcinoembryonic antigen (CEA) simultaneously.

中文翻译：

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基于 MXene 和磁性微球的高灵敏度“关-开”传感器，用于同时检测肺癌生物标志物 - 神经元特异性烯醇化酶和癌胚抗原

本工作基于Ag和MXene共功能化磁性微球开发了一种高灵敏度肺癌生物标志物检测探针。以羧基磁性微球为载体，以聚盐酸烯丙胺（PAH）为层间粘合剂反复包覆MXene，在MXene表面原位生长的银颗粒可有效提高探针的灵敏度。神经元特异性烯醇化酶（NSE）的检测主要是通过NSE抗原与抗体之间形成特异性复合物，并从银纳米粒子（AgNPs）表面释放氨基碳量子点（CQDs）标记的抗体，从而荧光恢复并形成“OFF-ON”。该生物传感器具有极宽的线性范围（0.0001-1500 ng/mL），检测限（LOD）高达0.03 pg/mL，优于大多数基于荧光机制的肿瘤标志物探针。此外，我们还构建了NSE和癌胚抗原（CEA）同时检测的策略。