The presence of zearalenone (ZEN) toxin in agricultural foods and animal feed ingredients has become a global public health concern due to its pathogenic effects. As a result, the development of novel, highly accurate, rapid, and reliable analytical methods for ZEN detection is essential for ensuring food safety. In the present work, a highly sensitive aptasensor for ZEN was developed using UiO-66-NH metal-organic framework (MOF) and graphene oxide (GO). A highly fluorescent UiO-66-NH MOF, conjugated with ZEN aptamer, was employed as a fluorescent signal probe (λ = 330/430 nm) while GO functioned as a quencher in the assay. In the presence of ZEN, the fluorescence intensity of the MOF increased significantly as the aptamer-UiO-66-NH showed its affinity toward ZEN, releasing the GO. Under the optimal experimental conditions, the assay displayed excellent specificity, repeatability, and linearity over the ZEN concentration range of 0.01–100 ng/mL, yielding a low detection limit of 5 pg/mL. In addition, the developed sensor also demonstrated satisfactory performance with recoveries from 85 to 114% in the spiked samples of wheat and corn flour. In general, the developed sensing strategy provides a new approach for the rapid, inexpensive, and sensitive fluorescent detection of ZEN in food matrices.

中文翻译：

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UiO-66-NH2 MOF 荧光适体传感器用于检测谷物中的玉米赤霉烯酮

农业食品和动物饲料成分中存在的玉米赤霉烯酮 (ZEN) 毒素因其致病作用已成为全球公共卫生问题。因此，开发新颖、高精度、快速且可靠的 ZEN 检测分析方法对于确保食品安全至关重要。在目前的工作中，使用 UiO-66-NH 金属有机框架 (MOF) 和氧化石墨烯 (GO) 开发了一种用于 ZEN 的高灵敏度适体传感器。与 ZEN 适体缀合的高荧光 UiO-66-NH MOF 被用作荧光信号探针 (λ = 330/430 nm)，而 GO 在测定中充当猝灭剂。在 ZEN 存在的情况下，MOF 的荧光强度显着增加，因为适体-UiO-66-NH 显示出对 ZEN 的亲和力，从而释放 GO。在最佳实验条件下，该检测在 0.01–100 ng/mL 的 ZEN 浓度范围内表现出出色的特异性、重复性和线性，检测限低至 5 pg/mL。此外，所开发的传感器还表现出令人满意的性能，在小麦和玉米粉的加标样品中回收率为 85% 至 114%。总的来说，所开发的传感策略为快速、廉价且灵敏地检测食品基质中的 ZEN 荧光提供了一种新方法。