A colorimetry/fluorescence dual-mode assay based on the aptamer-functionalized magnetic covalent organic framework-supported CuO and Au NPs (MCOF-CuO/Au@apt) was developed for () biosensing. The nanohybrid combined three functions in one: good magnetic separation characteristic, excellent oxidase-mimic activity for tetrap-aminophenylethylene (TPE-4A), and target recognition capability. The attachment of MCOF-CuO/Au@apt onto the surface of resulted in a significant reduction in the oxidase-mimicking activity of the nanohybrid, which could generate dual-signal of colorimetry and fluorescence through the catalytic oxidation of TPE-4A. Based on this, could be specifically detected in the linear ranges of 10– 10 CFU·mL and 10– 10 CFU·mL, with LODs of 7.6 and 2.1 CFU·mL, respectively in colorimetry/fluorescence modes. Moreover, the smartphone and linear discrimination analysis-based system could be used for on-site and portable testing. In addition, this platform showed applicability in detecting in milk, egg liquid and chicken samples.

中文翻译：

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基于AIEgen高氧化酶活性“三合一”纳米杂化物的鼠伤寒沙门氏菌比色/荧光双模式检测

开发了一种基于适配子功能化磁性共价有机框架支持的 CuO 和 Au NP (MCOF-CuO/Au@apt) 的比色/荧光双模式测定，用于 () 生物传感。该纳米杂化物将三种功能合二为一：良好的磁分离特性、优异的四氨基苯乙烯（TPE-4A）氧化酶模拟活性以及目标识别能力。将MCOF-CuO/Au@apt附着到纳米杂化物的表面会导致纳米杂化物的氧化酶模拟活性显着降低，从而可以通过TPE-4A的催化氧化产生比色和荧光的双信号。基于此，在比色/荧光模式下，可以在10–10 CFU·mL和10–10 CFU·mL的线性范围内特异性检测到，LOD分别为7.6和2.1 CFU·mL。此外，智能手机和基于线性判别分析的系统可用于现场和便携式测试。此外，该平台还适用于牛奶、蛋液和鸡肉样品的检测。