Metagenome assembled genomes (MAGs), generated from sequenced 13C‐labelled DNA from 13C‐methanol enriched soils, were binned using an ensemble approach. This method produced a significantly larger number of higher‐quality MAGs compared to direct binning approaches. These MAGs represent both the primary methanol utilizers and the secondary utilizers labelled via cross‐feeding and predation on the labelled methylotrophs, including numerous uncultivated taxa. Analysis of these MAGs enabled the identification of multiple metabolic pathways within these active taxa that have climatic relevance relating to nitrogen, sulfur and trace gas metabolism. This includes denitrification, dissimilatory nitrate reduction to ammonium, ammonia oxidation and metabolism of organic sulfur species. The binning of viral sequence data also yielded extensive viral MAGs, identifying active viral replication by both lytic and lysogenic phages within the methanol‐enriched soils. These MAGs represent a valuable resource for characterizing biogeochemical cycling within terrestrial environments.

中文翻译：

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基因组解析的宏基因组学鉴定了富含甲醇的土壤中生物地球化学循环中的新型活性微生物

宏基因组组装基因组 (MAG)，由测序生成13C标记的DNA来自13使用集成方法对富含 C-甲醇的土壤进行分类。与直接分箱方法相比，该方法产生了更多数量的高质量 MAG。这些 MAG 既代表甲醇的主要利用者，也代表通过交叉喂养和捕食标记的甲基营养生物（包括许多未培养的类群）标记的次级利用者。对这些 MAG 的分析能够识别这些活跃类群内的多种代谢途径，这些途径与氮、硫和痕量气体代谢具有气候相关性。这包括反硝化、异化硝酸盐还原成铵、氨氧化和有机硫物质的代谢。病毒序列数据的分箱还产生了广泛的病毒 MAG，识别了富含甲醇的土壤中裂解噬菌体和溶原噬菌体的活跃病毒复制。这些 MAG 是表征陆地环境中生物地球化学循环的宝贵资源。