The inappropriate use of antibiotics undoubtedly poses a potential threat to public health, creating an increasing need to develop highly sensitive tests. In this study, we designed a new type of porphyrin metal-organic frameworks (Fe TCPP(Zn) MOFs) with homogeneous catalytic sites. The ferric-based metal ligands of Fe TCPP(Zn) MOFs acted as co-reaction accelerators, which effectively improved the conversion efficiency of HO on the surface of MOFs, then increased the concentration of •OH surrounding porphyrin molecules to achieve self-enhanced electrochemiluminescence (ECL). Based on this, an aptasensor for the specific detection of kanamycin (KAN) in food and environmental water samples was constructed in combination with resonance energy transform (RET), in which Fe TCPP(Zn) MOFs were used as luminescence donor and AuNPs were used as acceptor. Under the best conditions, there was a good linear relationship between the ECL intensity and the logarithm of KAN concentration with a detection limit of 0.28 fM in the range of 1.0 × 10–1.0 × 10 M, demonstrating satisfactory selectivity and stability. At the same time, the complexity of the detection environment was reduced, which further realized the reliable analysis of KAN in milk, honey and pond water. Overall, this innovative self-enhanced ECL strategy provides a novel approach for constructing efficient ECL systems in MOFs, and also extends the application of MOFs to the analysis and detection of trace antibiotics in food and the environment.

中文翻译：

---

一种基于具有均质催化位点的双金属 MOF 的新型自增强 ECL-RET 适体传感器，用于卡那霉素检测

抗生素的不当使用无疑对公众健康构成了潜在威胁，因此越来越需要开发高灵敏度的检测方法。在这项研究中，我们设计了一种具有均质催化位点的新型卟啉金属有机框架（Fe TCPP(Zn) MOFs）。 Fe TCPP(Zn) MOFs的铁基金属配体作为共反应促进剂，有效提高了MOFs表面H2O的转化效率，进而增加卟啉分子周围的·OH浓度，实现自增强电化学发光（ECL）。在此基础上，以Fe TCPP(Zn) MOFs为发光供体，AuNPs为载体，结合共振能量转换(RET)技术，构建了特异性检测食品和环境水样中卡那霉素(KAN)的适配体传感器。作为接受者。在最佳条件下，ECL强度与KAN浓度对数之间存在良好的线性关系，在1.0×10~1.0×10 M范围内检测限为0.28 fM，表现出良好的选择性和稳定性。同时降低了检测环境的复杂度，进一步实现了牛奶、蜂蜜和池塘水中KAN的可靠分析。总体而言，这种创新的自我增强ECL策略为在MOF中构建高效ECL系统提供了一种新方法，并将MOF的应用扩展到食品和环境中痕量抗生素的分析和检测。