The largest () that LC analysis can generate in isocratic or gradient elution analysis of a given sample in a given time at a given pressure is proportional to the () of its column structure. In this study, the multi-channel structures with s (OPPC) and (OCC) where compared with PC2 – a typical core-shell packed with 2 μm particles. These columns have of 1.27, 1.17 and 0.41, respectively. The former two are the highest among all known column structures – about 3 times higher than of PC2. This means that the OPPC and OCC can generate about 3 times higher compared to what a PC2 can in the same analysis time () at the same pressure, or they require about 81 times shorter (81 is the 4th power of 3) to generate the same as a PC2 can at the same pressure. However, while PC2 is a commercially available column, there are substantial challenges in manufacturing the OPPC and OCC that can compete with PC2 in practical applications. In order to be competitive with PC2, the OPPC and OCC should have sub-1μm characteristic dimensions (e.g., the inter-pillar distance, , in OPPC-based pillar array columns, internal diameters of OCC). Thus, in order to compete with PC2 in one scenario, an OPPC requires g ≤ 0.14 μm. Additionally, to be competitive with PC2, OPPC and OCC should be able to sustain the same high pressure. Highlighting the challenges of their design and manufacturing might help to develop the manufacturable columns substantially superior to the packed ones.

中文翻译：

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液相色谱柱的最佳结构——理论视角

LC 分析在给定时间和给定压力下对给定样品进行等度或梯度洗脱分析时可产生的最大 () 与其色谱柱结构的 () 成正比。在这项研究中，具有 s (OPPC) 和 (OCC) 的多通道结构与 PC2（一种典型的填充有 2 μm 颗粒的核壳结构）进行了比较。这些列的值分别为 1.27、1.17 和 0.41。前两者是所有已知柱结构中最高的——大约是PC2的3倍。这意味着，与 PC2 相比，OPPC 和 OCC 在相同分析时间 ()、相同压力下生成的数据大约高出 3 倍，或者它们需要大约 81 倍的时间（81 是 3 的 4 次方）来生成与PC2相同，压力相同。然而，虽然PC2是一种商业化的色谱柱，但在制造可在实际应用中与PC2竞争的OPPC和OCC方面存在巨大的挑战。为了与 PC2 竞争，OPPC 和 OCC 应具有低于 1μm 的特征尺寸（例如，基于 OPPC 的柱阵列柱中的柱间距离、OCC 的内径）。因此，为了在一种情况下与 PC2 竞争，OPPC 需要 g ≤ 0.14 μm。此外，为了与PC2竞争，OPPC和OCC应该能够承受同样的高压。强调其设计和制造的挑战可能有助于开发可制造的色谱柱，其性能明显优于填充色谱柱。