Somatostatin (SST)-producing pancreatic delta-cells play an important role in maintaining the balance of insulin and glucagon secretion within the islets. This study aimed to generate a human embryonic stem cell (hESC) line with a SST-P2A-mCherry reporter using CRISPR/Cas9 system. The SST-P2A-mCherry reporter cell line was shown to maintain typical pluripotent characteristics and able to be induced into SST-producing pancreatic delta-cells. The generation of the cell line would provide useful platform for the characterization of stem cell-derived delta-cells, discovery of delta-cell surface markers and investigation of paracrine mechanisms, which will ultimately promote the drug discovery and cell therapy of diabetes mellitus.

中文翻译：

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使用 CRISPR/Cas9 系统生成 SST-P2A-mCherry 报告基因人胚胎干细胞系 (WAe001-A-2C)

产生生长抑素（SST）的胰腺δ细胞在维持胰岛内胰岛素和胰高血糖素分泌平衡方面发挥着重要作用。本研究旨在使用 CRISPR/Cas9 系统生成具有 SST-P2A-mCherry 报告基因的人类胚胎干细胞 (hESC) 系。 SST-P2A-mCherry 报告细胞系显示出保持典型的多能特性，并且能够被诱导成产生 SST 的胰腺 delta 细胞。该细胞系的产生将为干细胞衍生的δ细胞的表征、δ细胞表面标记的发现和旁分泌机制的研究提供有用的平台，这最终将促进糖尿病的药物发现和细胞治疗。