The accurate detection of biogenic amines (BAs) is an important means of ensuring the quality and safety of cephalopod seafood products. In this study, the pre‐column derivatization of high‐performance liquid chromatography (HPLC) was optimized using dansyl chloride (Dns‐Cl) to detect BAs in octopus, cuttlefish, and squid. The reasons for the formation of BAs were investigated by assessing their decarboxylase activity and the rates of decomposition. The findings demonstrated that using Dns‐Cl to optimize pre‐column derivatization enabled the separation of nine different BAs. The detection limits ranged from 0.07 to 0.25 mg/L, and the results exhibited a high level of linearity (R2 ≥ 0.997). The decarboxylase activity and biodegradation rate positively correlated with the formation of BAs at temperatures below 0°C. Notably, the decarboxylase activity of octopus, cuttlefish, and squid exhibited a significant increase with prolonged storage time, and formyltransferase and carbamate kinase may be the key decarboxylase in cephalopod products. These findings serve as a valuable reference for further investigations into the mechanisms behind BAs production and the development of control technologies for BAs in cephalopod products. This study has successfully demonstrated the effectiveness of the Dns‐Cl pre‐column derivatization‐HPLC method in accurately and efficiently detecting BAs in octopus, cuttlefish, and squid. Moreover, it highlights the influence of decarboxylase content and biodegradation rate on the formation of BAs. Importantly, this method can serve as a reference for detecting BAs in various seafood products.

中文翻译：

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丹磺酰氯柱前衍生高效液相色谱法检测和定量头足类生物胺及其制备

生物胺（BAs）的准确检测是保证头足类海产品质量安全的重要手段。在本研究中，使用丹磺酰氯 (Dns-Cl) 优化了高效液相色谱 (HPLC) 的柱前衍生化，以检测章鱼、墨鱼和鱿鱼中的 BA。通过评估 BA 的脱羧酶活性和分解速率，研究了 BA 形成的原因。研究结果表明，使用 Dns-Cl 优化柱前衍生化能够分离九种不同的 BA。检测限范围为0.07~0.25 mg/L，结果呈现出较高的线性度（右2≥0.997）。在低于0°C的温度下，脱羧酶活性和生物降解率与BAs的形成呈正相关。值得注意的是，章鱼、墨鱼和鱿鱼的脱羧酶活性随着储存时间的延长而显着增加，甲酰基转移酶和氨基甲酸酯激酶可能是头足类产品中的关键脱羧酶。这些研究结果为进一步研究头足类产品中BAs的产生机制和开发BAs控制技术提供了有价值的参考。本研究成功证明了 Dns-Cl 柱前衍生化 HPLC 方法在准确、高效检测章鱼、墨鱼和鱿鱼中 BA 的有效性。此外，它还强调了脱羧酶含量和生物降解率对BAs形成的影响。重要的是，该方法可以作为检测各种海鲜产品中BAs的参考。