Pathogenic fungi pose a significant threat to crop yields and human healthy, and the subsequent fungicide resistance has greatly aggravated these agricultural and medical challenges. Hence, the development of new fungicides with higher efficiency and greater environmental friendliness is urgently required. In this study, luvangetin, isolated and identified from the root of Zanthoxylum avicennae, exhibited wide-spectrum antifungal activity in vivo and in vitro. Integrated omics and in vitro and in vivo transcriptional analyses revealed that luvangetin inhibited GAL4-like Zn(II)₂Cys6 transcriptional factor-mediated transcription, particularly the FvFUM21-mediated FUM cluster gene expression, and decreased the biosynthesis of fumonisins inFusarium verticillioides. Moreover, luvangetin binds to the double-stranded DNA helix in vitro in the groove mode. We isolated and identified luvangetin, a natural metabolite from a traditional Chinese edible medicinal plant and uncovered its multipathogen resistance mechanism. This study is the first to reveal the mechanism underlying the antifungal activity of luvangetin and provides a promising direction for the future use of plant-derived natural products to prevent and control plant and animal pathogenic fungi.

中文翻译：

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花椒中 Luvangetin 的发现和开发作为轮枝镰刀菌新候选杀菌剂

病原真菌对农作物产量和人类健康构成重大威胁，随后的杀菌剂抗性极大地加剧了这些农业和医学挑战。因此，迫切需要开发更高效、更环保的新型杀菌剂。在这项研究中，从Zanthoxylum avicennae的根中分离和鉴定的luvangetin在体内和体外表现出广谱抗真菌活性。综合组学和体内外转录分析表明，luvangetin 抑制 GAL4 样 Zn(II) ₂ Cys6 转录因子介导的转录，特别是 FvFUM21 介导的FUM簇基因表达，并减少轮枝镰刀菌中伏马菌素的生物合成。此外，luvangetin在体外以凹槽模式与双链 DNA 螺旋结合。我们分离鉴定了一种传统中药用植物的天然代谢产物卢万盖汀，并揭示了其多病原体抗性机制。该研究首次揭示了luvangetin抗真菌活性的机制，为未来利用植物源天然产物防治动植物病原真菌提供了一个有前景的方向。