Chlorination is the common antifouling method in desalination and power plant water intake structures to control microbial and macrofouling growth. In this study, the impacts of chlorine dioxide on toxicity, metabolic activity and biochemical markers like glutathione S-transferase and catalase enzyme activity were tested using four zooplankton species ( sp., sp., sp., and sp.) collected from the Red Sea. The zooplankton species were treated with different concentrations (0.02, 0.05, 0.1, 0.2, and 0.5 mg L) of chlorine dioxide. Further, chlorite, the main decomposition product of chlorine dioxide, was used for molecular docking studies against glutathione S-transferase and catalase enzymes. The results indicated the LC range of 0.552–1.643 mg L for the studied zooplankton species. The respiration rate of the zooplankton increased due to the chlorine dioxide treatment with a maximum of 0.562 μg O copepod h in . The glutathione S-transferase and catalase enzyme activities showed elevated values in zooplankton treated with chlorine dioxide. Molecular docking of chlorite with enzymes involved in antioxidant defense activity, such as glutathione S-transferase and catalase enzyme showed weak interactions. Overall, this study yielded significant insights for understanding the effects of chlorine dioxide on the survival, metabolism, and biochemical composition of marine zooplankton.

中文翻译：

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毒性测定和计算机模拟方法评估二氧化氯对海洋浮游动物的生存、呼吸和一些生化标志物的影响

氯化是海水淡化和发电厂取水结构中常用的防污方法，用于控制微生物和大型污垢的生长。在本研究中，使用从红海中收集的四种浮游动物物种（sp.、sp.、sp. 和 sp.）测试了二氧化氯对毒性、代谢活性和生化标志物（如谷胱甘肽 S-转移酶和过氧化氢酶活性）的影响。海。用不同浓度（0.02、0.05、0.1、0.2 和 0.5 mg·L）的二氧化氯处理浮游动物物种。此外，二氧化氯的主要分解产物亚氯酸盐被用于针对谷胱甘肽S-转移酶和过氧化氢酶的分子对接研究。结果表明，所研究的浮游动物物种的 LC 范围为 0.552–1.643 mg·L。由于二氧化氯处理，浮游动物的呼吸速率增加，桡足类的最大浓度为 0.562 μg O·h。用二氧化氯处理的浮游动物的谷胱甘肽 S-转移酶和过氧化氢酶活性显示出升高的值。亚氯酸盐与参与抗氧化防御活性的酶（例如谷胱甘肽 S-转移酶和过氧化氢酶）的分子对接显示出弱相互作用。总体而言，这项研究为了解二氧化氯对海洋浮游动物的生存、代谢和生化成分的影响提供了重要的见解。