Fusarium wilt is a severe fungal disease caused by Fusarium oxysporum in sweet potato. We conducted transcriptome analysis to explore the resistance mechanism of sweet potato against F. oxysporum. Our findings highlighted the role of scopoletin, a hydroxycoumarin, in enhancing resistance. In vitro experiments confirmed that scopoletin and umbelliferone had inhibitory effects on the F. oxysporum growth. We identified hydroxycoumarin synthase genes IbF6′H2 and IbCOSY that are responsible for scopoletin production in sweet potatoes. The co-overexpression of IbF6′H2 and IbCOSY in tobacco plants produced the highest scopoletin levels and disease resistance. This study provides insights into the molecular basis of sweet potato defense against Fusarium wilt and identifies valuable genes for breeding wilt-resistant cultivars.

中文翻译：

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甘薯中东莨菪素的生物合成赋予对尖孢镰刀菌的抗性

甘薯枯萎病是由尖孢镰刀菌引起的一种严重的甘薯真菌病害。我们通过转录组分析来探讨甘薯对尖孢镰刀菌的抗性机制。我们的研究结果强调了东莨菪素（一种羟基香豆素）在增强抵抗力方面的作用。体外实验证实东莨菪素和伞形酮对尖孢镰刀菌的生长有抑制作用。我们鉴定了负责甘薯中东莨菪素生产的羟基香豆素合酶基因IbF6'H2和IbCOSY 。烟草植物中IbF6'H2和IbCOSY的共过表达产生了最高的东莨菪素水平和抗病性。这项研究提供了甘薯防御镰刀菌枯萎病的分子基础的见解，并确定了培育抗枯萎病品种的有价值的基因。