Cyclic polymers, which are found in the field of biopolymers, exhibit unique physical properties such as suppressed molecular mobility. Considering thermodynamics, the suppressed molecular mobility of cyclic polymers is expected to prevent unfavorable entropy loss in molecular interactions. In this study, we synthesized cyclic glycopolymers carrying galactose units and investigated the effects of their molecular mobility on the interactions with a lectin (peanut agglutinin). The synthesized cyclic glycopolymers exhibited delayed elution time on size exclusion chromatography and a short spin–spin relaxation time, indicating typical characteristics of cyclic polymers, including smaller hydrodynamic size and suppressed molecular mobility. The hemagglutination inhibition assay revealed that the cyclic glycopolymers exhibited weakened interactions with PNA compared to the linear counterparts, attributable to the suppressed molecular mobility. Although the results are contrary to our expectations, the impact of polymer topology on molecular recognition remains intriguing, particularly in the context of protein repellent activity in the biomedical field.

中文翻译：

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环状糖聚合物分子迁移率对其与凝集素相互作用的影响

生物聚合物领域中发现的环状聚合物表现出独特的物理性质，例如抑制分子运动性。考虑到热力学，环状聚合物的抑制分子运动性有望防止分子相互作用中不利的熵损失。在这项研究中，我们合成了携带半乳糖单元的环状糖聚合物，并研究了它们的分子迁移率对与凝集素（花生凝集素）相互作用的影响。合成的环状糖聚合物在尺寸排阻色谱上表现出延迟的洗脱时间和短的自旋-自旋弛豫时间，表明环状聚合物的典型特征，包括较小的流体动力学尺寸和抑制的分子迁移率。血凝抑制测定表明，与线性对应物相比，环状糖聚合物与 PNA 的相互作用减弱，这归因于分子迁移率受到抑制。尽管结果与我们的预期相反，但聚合物拓扑结构对分子识别的影响仍然令人着迷，特别是在生物医学领域的蛋白质排斥活性背景下。