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Study on the effect of different high-voltage electric field polarization process parameters on the vitality of dried chili pepper seeds
Scientific Reports ( IF 4.6 ) Pub Date : 2024-03-27 , DOI: 10.1038/s41598-024-57978-z
Sheng Sun , Bin Hu , Xinming Wu , Xin Luo , Mengyu Guo , Hanjun Liu

To study the effect of different high-voltage electric field polarisation treatment process parameters on the viability of seeds of dried chili peppers. In this study, a high-voltage electrostatic polarisation treatment system was constructed to carry out experiments on the effects of different high-voltage electric field polarisation treatment process parameters on the viability of dried chili seeds. Conduct one-way tests to determine the preferred polarisation method and the preferred interval for output voltage and polarisation time. Two-factor, five-level central combination test with output voltage and polarization time as test factors and seed conductivity as a response indicator. Determining the better combination of parameters for output voltage and polarization time; Conducting seed germination trials to validate the effectiveness of the polarisation process. The results of the one-way test showed that: Negative-voltage polarisation was more effective than positive-voltage polarisation and alternating positive–negative-voltage polarisation in promoting seed vigor, with a better output voltage in the range of 10–14 kV, and a better polarisation time in the range of 20–40 s; The results of orthogonal tests showed that: Under the condition of negative voltage polarisation treatment, the output voltage of 12.08 kV and polarisation time of 30.32 s was the better parameter combination, at which the seed conductivity was minimum 159.87 uS/(cm g). Analyzing the function of cell membrane selective semi-permeability by seed conductivity change and revealing the mechanism of seed viability enhancement by high voltage electric field polarisation treatment; In the seed germination test, compared with the control group, seed germination potential increased by 9.09%, germination rate increased by 20.45%, germination index increased by 3.49, and vigor index increased by 41.66 under high-voltage electrostatic polarisation treatment, and all vigor indexes were significantly improved. The results of this study can provide a basis for the selection of processes and parameters for subsequent high-voltage electric field polarisation treatment of crop seeds.



中文翻译:

不同高压电场极化工艺参数对干辣椒种子活力影响的研究

研究不同高压电场极化处理工艺参数对干辣椒种子活力的影响。本研究搭建高压静电极化处理系统,开展不同高压电场极化处理工艺参数对干辣椒种子活力影响的实验。进行单向测试以确定优选的极化方法以及输出电压和极化时间的优选间隔。以输出电压和极化时间为测试因素,种子电导率作为响应指标的二因素五级中心组合测试。确定输出电压和极化时间的较好参数组合;进行种子发芽试验以验证极化过程的有效性。单向试验结果表明:负电压极化比正电压极化和正负电压交替极化对种子活力的促进效果更好,在10~14 kV范围内输出电压较好,更好的极化时间在20-40秒范围内;正交试验结果表明:负电压极化处理条件下,输出电压12.08 kV、极化时间30.32 s是较好的参数组合,此时种子电导率最小,为159.87 uS/(cm·g)。通过种子电导率变化分析细胞膜选择性半透性的作用,揭示高压电场极化处理增强种子活力的机制;种子发芽试验中,与对照组相比,高压静电极化处理下种子发芽势提高9.09%,发芽率提高20.45%,发芽指数提高3.49,活力指数提高41.66,全部活力各项指标均显着提高。本研究结果可为后续作物种子高压电场极化处理工艺及参数选择提供依据。

更新日期:2024-03-27
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