ObjectiveDichlorodiphenyldichloroethylene (DDE), an obesogen accumulating in adipose tissue, is released into circulation with weight loss, although its impact is underexplored among adolescents. We tested the association using an integrative translational approach of epidemiological analysis among adolescents with obesity and in vitro measures exploring the impact of DDE on adipogenesis via preadipocytes.MethodsWe included 63 participants from the Teen‐Longitudinal Assessment of Bariatric Surgery (Teen‐LABS) cohort. We assessed 4,4′‐DDE in visceral adipose tissue at surgery and BMI and waist circumference at surgery and 0.5, 1, 3, and 5 years after. We conducted longitudinal analysis to estimate the interaction on weight loss between DDE and time since surgery. In vitro analysis quantified adipogenic differentiation in commercial human preadipocytes exposed to 4,4′‐DDE via fluorescent staining and imaging.ResultsA dose–response relationship was observed, with the low‐exposure group having a greater reduction in BMI during the first year compared to higher‐exposure groups and showing smaller regains compared to higher‐exposure groups after the first year. In vitro analysis of preadipocytes treated with 4,4′‐DDE during adipogenic differentiation for 12 days showed a concentration‐dependent increase in lipid accumulation.ConclusionsDDE could contribute to weight trajectory among adolescents undergoing bariatric surgery, potentially mediated via promoted adipogenesis in preadipocytes.

中文翻译：

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Teen-LABS 队列青少年内脏脂肪组织中暴露于 4,4′-DDE 和体重减轻

目的 二氯二苯基二氯乙烯 (DDE) 是一种在脂肪组织中积累的肥胖物质，随着体重减轻而释放到循环系统中，尽管其影响在青少年中尚未得到充分研究。我们使用流行病学分析的综合转化方法在肥胖和肥胖青少年中测试了这种关联。体外探索 DDE 通过前脂肪细胞对脂肪生成的影响的措施。方法我们纳入了来自青少年减肥手术纵向评估 (Teen-LABS) 队列的 63 名参与者。我们评估了手术时内脏脂肪组织中的 4,4'-DDE，以及手术时和术后 0.5、1、3 和 5 年的 BMI 和腰围。我们进行了纵向分析来估计 DDE 与手术后时间之间体重减轻的相互作用。体外分析通过荧光染色和成像量化了暴露于 4,4'-DDE 的商业人类前脂肪细胞的脂肪形成分化。结果观察到剂量-反应关系，与高暴露组相比，低暴露组在第一年的 BMI 降低幅度更大。与高暴露组相比，暴露组在第一年后表现出较小的恢复。体外对在脂肪形成分化期间用 4,4'-DDE 处理的前脂肪细胞 12 天的分析显示，脂质积累呈浓度依赖性增加。 结论 DDE 可能有助于接受减肥手术的青少年的体重轨迹，可能是通过促进前脂肪细胞中的脂肪形成来介导的。