Gold complexes have shown great potential for clinical applications in cancer chemotherapy. However, the unclear mechanisms of tumor heterogeneity and induced drug resistance hinder the effectiveness of gold complex therapy. To address this issue, single-cell electrochemical detection strategies have been widely reported for bioimaging and bioanalysis, due to their in-situ, real-time, and high-resolution characteristics. In this study, the self-assembled process of gold complexes was investigated by detecting hydrogen peroxide release at the single-cell level using nanoelectrodes and scanning electrochemical microscopy. This detection strategy could provide more spatio-temporal information on the synthesis of bioresponsive fluorescent nanoprobes.

中文翻译：

---

基于单细胞电化学的细胞内原位自组装金配合物的双峰分析

金配合物在癌症化疗的临床应用中显示出巨大的潜力。然而，肿瘤异质性和诱导耐药性的机制尚不清楚，阻碍了金络合物治疗的有效性。为了解决这个问题，单细胞电化学检测策略因其原位、实时和高分辨率的特性而被广泛报道用于生物成像和生物分析。在这项研究中，通过使用纳米电极和扫描电化学显微镜检测单细胞水平的过氧化氢释放来研究金配合物的自组装过程。这种检测策略可以为生物响应荧光纳米探针的合成提供更多的时空信息。