A novel arylalkylamine N-acetyltransferase (AANAT) was identified and rationally engineered to improve the catalytic activity. The resultant mutant was then utilized in the transformation of 5-hydroxytryptamine (5-HT) to N-acetyl-5-hydroxytryptamine (NAS), enabling a 99 % conversion and an 80 % yield.

中文翻译：

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工程设计新鉴定的芳基烷基胺 N-乙酰基转移酶在 5-羟色胺乙酰化中的活性

鉴定出一种新型芳基烷基胺N-乙酰转移酶（AANAT），并对其进行合理改造以提高催化活性。然后将所得突变体用于将 5-羟​​色胺 (5-HT) 转化为N-乙酰基-5-羟色胺 (NAS)，实现 99% 的转化率和 80% 的产率。