Organic food consumption in children has been shown to reduce the body burden of chemical pesticides. However, there is little evidence of human health benefits associated with the consumption of organic foods. The objectives were to i) determine the effectiveness of an organic food intervention treatment in reducing the magnitude of an inflammation biomarker (C-reactive protein, CRP) in children (10–12 years) and ii) assess the association between the urinary biomarkers of exposure to pesticides and CRP. This work was part of the ORGANIKO cluster-randomized cross-over trial entailing a 40-day organic food treatment in healthy children. Urinary biomarkers of exposure to pesticides and inflammation (CRP) were measured using tandem mass spectrometry and ELISA immunoassay, respectively. Linear mixed-effect regression models of CRP were used to account for the effect and duration of organic food treatment. Multiple comparisons were handled using Benjamini-Hochberg correction. Results supported an anti-inflammatory effect of organic food treatment in children, albeit with mixed results, depending on the creatinine adjustment method; biomarker levels were divided by urinary creatinine (method a1), or urinary creatinine was used as a fixed effect variable (a2). In the a1 method, a time-dependent reduction for creatinine-adjusted CRP (β = −0.019; 95% CI: −0.031, −0.006; = 0.045) was observed during the organic food intervention period. A statistically significant association (β = 0.104; 95% CI: 0.035, 0.173; = 0.045) was found between the biomarker of pyrethroids exposure (3-PBA) and CRP inflammatory biomarker, but not for 6-CN. In the a2 method, similar trend of time-dependent reduction for creatinine-adjusted CRP (β = −0.008; 95% CI: −0.021, 0.004; = 0.197) was observed during the organic food intervention period, but did not reach statistical significance (q > 0.05); the associations of pyrethroid and neonicotinoid biomarkers with CRP were not statistically significant (q > 0.05). More studies are warranted to sufficiently understand the potential anti-inflammatory response of an organic food treatment.

中文翻译：

---

通过减少塞浦路斯儿童农药暴露来进行有机食品干预的抗炎反应：整群随机交叉试验


儿童食用有机食品已被证明可以减少化学农药对身体的负担。然而，几乎没有证据表明食用有机食品对人类健康有益。目标是 i) 确定有机食品干预治疗在降低儿童（10-12 岁）炎症生物标志物（C 反应蛋白，CRP）强度方面的有效性，以及 ii) 评估以下尿液生物标志物之间的关联：接触农药和 CRP。这项工作是 ORGANIKO 整群随机交叉试验的一部分，该试验需要对健康儿童进行为期 40 天的有机食品治疗。分别使用串联质谱法和 ELISA 免疫测定法测量农药暴露和炎症 (CRP) 的尿液生物标志物。 CRP 的线性混合效应回归模型用于解释有机食品处理的效果和持续时间。使用 Benjamini-Hochberg 校正进行多重比较。结果支持有机食品治疗对儿童具有抗炎作用，尽管结果好坏参半，具体取决于肌酐调整方法；生物标志物水平除以尿肌酐（方法 a1），或将尿肌酐用作固定效应变量（a2）。在a1方法中，在有机食品干预期间观察到肌酐调整的CRP随时间的减少（β = -0.019；95% CI：-0.031，-0.006；= 0.045）。拟除虫菊酯暴露生物标志物 (3-PBA) 与 CRP 炎症生物标志物之间存在统计学显着关联（β = 0.104；95% CI：0.035、0.173；= 0.045），但 6-CN 则不然。在 a2 方法中，肌酐调整的 CRP 也有类似的时间依赖性降低趋势（β = -0.008；95% CI：-0.021, 0.004；= 0。197）在有机食品干预期间观察到，但未达到统计学显着性（q > 0.05）；拟除虫菊酯和新烟碱类生物标志物与 CRP 的相关性不具有统计学意义 (q > 0.05)。需要更多的研究来充分了解有机食品治疗的潜在抗炎反应。