Persimmon ( L. cv. Mopan.), an important commercial crop belonging to the genus of in the Ebenaceae family, is rich in bioactive phenolic compounds. In this study, the phenolic compounds from fruits, leaves, and calyces of persimmon were qualitatively and quantitatively determined by UPLC-Q-Exactive-Orbitrap/MS and UPLC-QqQ-MS/MS, respectively. Furthermore, the role of phenolic extract from different parts of persimmon on neuroprotective activity , through against oxidative stress and anti-neuroinflammation effect was firstly evaluated. The results showed that 75 phenolic compounds, and 3 other kinds of compounds were identified, among which 44 of phenolic compounds were quantified from different parts of persimmon. It is the first time that epicatechin-epigallocatechin, catechin-epigallocatechin, catechin-epigallocatechin (A-type), and glycoside derivatives of laricitrin were identified in persimmon extract. The dominated phenolic compounds in three parts of persimmon were significantly different. All phenolic extracts from each part of persimmon showed strong neuroprotective activities against HO-induced oxidative stress in PC-12 cells and LPS-induced BV2 cells. The fruit extract presented the strongest activity, followed by calyx and leaf extract. The systematic knowledge on the phytochemical composition along with activity evaluation of different parts of persimmon could contribute to their targeted selection and development.

中文翻译：

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柿子不同部位酚类化合物UHPLC-MS/MS分析及对神经退行性疾病的保护作用磨盘

柿子（L. cv. Mopan.）是石榴科柿属的重要经济作物，富含具有生物活性的酚类化合物。本研究分别采用UPLC-Q-Exactive-Orbitrap/MS和UPLC-QqQ-MS/MS对柿果实、叶和花萼中的酚类化合物进行定性和定量测定。此外，首次评估了柿子不同部位的酚提取物通过抗氧化应激和抗神经炎症作用的神经保护活性作用。结果表明，共鉴定出75种酚类化合物和3种其他化合物，其中从柿子的不同部位定量出44种酚类化合物。首次在柿子提取物中鉴定出表儿茶素-表没食子儿茶素、儿茶素-表没食子儿茶素、儿茶素-表没食子儿茶素（A型）和落叶松苷的糖苷衍生物。柿子三个部位的主要酚类化合物差异显着。柿子各部分的所有酚类提取物均对 PC-12 细胞和 LPS 诱导的 BV2 细胞中 H2O2 诱导的氧化应激表现出强大的神经保护活性。果实提取物的活性最强，其次是花萼和叶提取物。对柿子植物化学成分的系统了解以及对柿子不同部位的活性评价有助于其有针对性的选择和开发。