Initially dismissed as ‘junk DNA’, genomic progress highlights the essential role of non-coding regulatory RNAs (ncRNAs) in gene expression. Long non-coding RNA (lncRNAs) and microRNA (miRNAs) play a vital role in regulating diverse plant biological processes. Despite extensive identification and characterization through bioinformatics and RNA-seq, only a limited subset of lncRNAs has been functionally studied. In this particular research, a total of twenty-six drought induced lncRNAs were selected from RNA-seq dataset of for in depth analysis including promoter analysis and the examination of their expression patterns under abiotic stress conditions, with the aim of unravelling their intricate regulatory mechanisms. Promoter analysis revealed their responsiveness to both ABA-independent and dependent pathways, indicating potential roles in pathway-regulated responses. Notably, three lncRNAs- and found to act as ceRNA (competing endogenous RNAs) for family members and respectively. Subsequent target prediction revealed that family members target , Osa- targets and targets precursor genes. Furthermore, qRT-PCR was employed to investigate the expression patterns and target mimicry mechanisms of selected lncRNAs under various abiotic stresses, revealing temporally regulated expressions. This finding provides insights into plant regulatory mechanisms under environmental stress, emphasizing the potential importance of lncRNAs in these processes.

中文翻译：

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目标拟态：水稻非生物胁迫反应复杂网络中 LncRNA 和 miRNA 的相互作用

基因组进展最初被视为“垃圾 DNA”，但现在凸显了非编码调节 RNA (ncRNA) 在基因表达中的重要作用。长链非编码RNA (lncRNA) 和微小RNA (miRNA) 在调节多种植物生物过程中发挥着至关重要的作用。尽管通过生物信息学和 RNA-seq 进行了广泛的鉴定和表征，但仅对有限的 lncRNA 子集进行了功能研究。在这项特殊的研究中，从RNA-seq数据集中总共选择了26个干旱诱导的lncRNA进行深入分析，包括启动子分析和检查它们在非生物胁迫条件下的表达模式，目的是揭示它们复杂的调控机制。启动子分析揭示了它们对 ABA 独立和依赖性途径的反应，表明在途径调节反应中的潜在作用。值得注意的是，三个 lncRNA- 和 被发现分别充当家族成员 和 的 ceRNA（竞争性内源 RNA）。随后的目标预测表明，家族成员以 、Osa- 为目标，并以前体基因为目标。此外，采用qRT-PCR研究了选定的lncRNA在各种非生物胁迫下的表达模式和靶向模拟机制，揭示了时间调控的表达。这一发现提供了对环境胁迫下植物调控机制的见解，强调了 lncRNA 在这些过程中的潜在重要性。