To explore the mechanism by which Remazolam affects the phenotype and function of astrocytes to improve traumatic brain injury (TBI). The oxygen -glucose deprivation/recovery (OGD/R) cell model was constructed to simulate the pathological state of astrocytes in a TBI environment. The viability of astrocytes was measured by CCK-8, and the cytoskeleton changes were observed by Phalloidin- TRITC staining. The expressions of differentiation markers, Cx43 and phosphorylated Cx43 (P-Cx43) of A1/A2 astrocytes were detected by Western blot, and the complement C3 and S100A10 of A1/A2 astrocytes were detected by ELISA. The TBI rat model was established. The water content of brain tissue was measured by dry-wet specific gravity method, the pathological morphology of brain tissue in cortical injury area was observed by HE staining method, ROS was detected by fluorescence quantitative method, Cx43 expression was detected by immunohistochemistry method, and the differentiation markers of A1/A2 astrocytes were detected by immunofluorescence. In the TBI environment, astrocytes showed decreased cell viability, blurred skeleton, and increased expression of Cx43. In TBI rats, the water content of brain tissue increased, the brain tissue in the cortex injury area was seriously damaged, ROS and Cx43 expression were significantly increased, and mainly distributed in A2 astrocytes. Remazolam can reverse the above results after the intervention. Remazolam affects the phenotype and function of astrocytes to improve TBI via regulating Cx43, and plays a role in protecting the neurological function of TBI rats.

中文翻译：

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雷马唑仑影响星形胶质细胞的表型和功能，通过调节 Cx43 改善创伤性脑损伤

探讨雷马唑仑影响星形胶质细胞表型和功能以改善创伤性脑损伤（TBI）的机制。构建氧糖剥夺/恢复（OGD/R）细胞模型来模拟TBI环境中星形胶质细胞的病理状态。采用CCK-8测定星形胶质细胞活力，鬼笔环肽-TRITC染色观察细胞骨架变化。 Western blot检测A1/A2星形胶质细胞分化标志物Cx43和磷酸化Cx43（P-Cx43）的表达，ELISA检测A1/A2星形胶质细胞补体C3和S100A10的表达。建立TBI大鼠模型。采用干湿比重法测定脑组织含水量，HE染色法观察皮质损伤区脑组织病理形态，荧光定量法检测ROS，免疫组化法检测Cx43表达，免疫荧光检测A1/A2星形胶质细胞分化标志物。在 TBI 环境中，星形胶质细胞表现出细胞活力下降、骨架模糊以及 Cx43 表达增加。 TBI大鼠脑组织含水量增加，皮质损伤区脑组织损伤严重，ROS、Cx43表达显着升高，主要分布于A2星形胶质细胞。雷马唑仑干预后可以逆转上述结果。雷马唑仑通过调节Cx43影响星形胶质细胞的表型和功能改善TBI，对TBI大鼠的神经功能起到保护作用。