Isothermal titration calorimetry (ITC) and surface plasmon resonance (SPR) are two commonly used methods to probe biomolecular interactions. ITC can provide information about the binding affinity, stoichiometry, changes in Gibbs free energy, enthalpy, entropy, and heat capacity upon binding. SPR can provide information about the association and dissociation kinetics, binding affinity, and stoichiometry. Both methods can determine the nature of protein–protein interactions and help understand the physicochemical principles underlying complex biochemical pathways and communication networks. This methods article discusses the practical knowledge of how to set up and troubleshoot these two experiments with some examples.

中文翻译：

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等温滴定量热法和表面等离子共振方法探测蛋白质-蛋白质相互作用

等温滴定量热法 (ITC) 和表面等离子体共振 (SPR) 是探测生物分子相互作用的两种常用方法。 ITC 可以提供有关结合亲和力、化学计量、结合时吉布斯自由能、焓、熵和热容变化的信息。 SPR 可以提供有关缔合和解离动力学、结合亲和力和化学计量的信息。这两种方法都可以确定蛋白质-蛋白质相互作用的性质，并有助于理解复杂生化途径和通讯网络背后的物理化学原理。本方法文章通过一些示例讨论了如何设置这两个实验并排除故障的实用知识。