Vascular cells form a highly complex and heterogeneous tissue. Its composition, function, shape, and arrangement vary with the developmental stage and between organs and species. Understanding the transcriptional regulation underpinning this complexity thus requires a high-resolution technique that is capable of capturing rapid events during vascular cell formation. Single-cell and single-nucleus RNA sequencing (sc/snRNA-seq) approaches provide powerful tools to extract transcriptional information from these lowly abundant and dynamically changing cell types, which allows the reconstruction of developmental trajectories. Here, we summarize and reflect on recent studies using single-cell transcriptomics to study vascular cell types and discuss current and future implementations of sc/snRNA-seq approaches in the field of vascular development.

中文翻译：

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使用单细胞方法研究植物维管发育

血管细胞形成高度复杂和异质的组织。它的组成、功能、形状和排列随着发育阶段以及器官和物种的不同而变化。因此，了解支撑这种复杂性的转录调控需要一种高分辨率技术，能够捕获血管细胞形成过程中的快速事件。单细胞和单核 RNA 测序 (sc/snRNA-seq) 方法提供了强大的工具，可以从这些低丰度且动态变化的细胞类型中提取转录信息，从而重建发育轨迹。在这里，我们总结并反思了最近使用单细胞转录组学研究血管细胞类型的研究，并讨论了 sc/snRNA-seq 方法在血管发育领域当前和未来的实施。