The extensive use of antidiabetic drugs (ADDs) and their detection in high concentrations in the environment have been extensively documented. However, the mechanism of ADDs dissipation in aquatic environments is still not well understood. This study thoroughly investigates the dissipation behavior of ADDs and the underlying mechanisms in the aerobic activated sludge system. The results indicate that the removal efficiencies of ADDs range from 3.98% to 100% within 48 h, largely due to the biodegradation process. Additionally, the gene expression of cytochrome P450 (CYP450) is shown to be significantly upregulated in most ADDs-polluted samples (P < 0.05), indicating the vital role of CYP450 enzymes in the biodegradation of ADDs. Enzyme inhibition experiments validated this hypothesis. Moreover, molecular docking and simulation results indicate that a strong correlation between the biodegradation of ADDs and the interactions between ADDs and CYP450 (E). The differences in dissipation behavior among the tested ADDs are possibly due to their electrophilic characteristics. Overall, this study makes the initial contribution to a more profound comprehension of the crucial function of CYP450 enzymes in the dissipation behavior of ADDs in a typical aquatic environment.

中文翻译：

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CYP450在好氧活性污泥系统抗糖尿病药物生物降解中的重要作用及其机制

抗糖尿病药物 (ADD) 的广泛使用及其在环境中高浓度的检测已得到广泛记录。然而，ADDs在水生环境中消散的机制仍不清楚。本研究深入研究了好氧活性污泥系统中 ADD 的消散行为及其潜在机制。结果表明，ADDs 的去除效率在 48 小时内达到 3.98% 至 100%，这主要归功于生物降解过程。此外，在大多数 ADDs 污染的样品中，细胞色素 P450 (CYP450) 的基因表达显着上调 (P < 0.05)，表明 CYP450 酶在 ADDs 生物降解中的重要作用。酶抑制实验验证了这一假设。此外，分子对接和模拟结果表明ADDs的生物降解与ADDs和CYP450之间的相互作用之间存在很强的相关性(E)。所测试的 ADD 耗散行为的差异可能是由于它们的亲电子特性造成的。总的来说，这项研究为更深入地理解 CYP450 酶在典型水生环境中 ADD 消散行为中的关键功能做出了初步贡献。