An oily bee pollen extract was obtained using the supercritical fluid technique (SFE) using CO2 as a solvent and conditions of pressure: 28 MPa, temperature: 60°C, gas flow: 5 L/min, and duration: 6 h. Initially, pollen samples were collected from different municipalities of Colombia, selected, and analyzed according to the content of total carotenoids (CCT) and yield of the extraction process. The results show CCT between 119 and 2140 µg β-carotene/g of sample, with yield percentages in obtaining oily extracts between 3 – 5%. For CCT in the oily extract, 385–6942 µg β-carotene/g of extract were obtained, where Guatavita pollen was the one with the highest content. According to the investigation, the authors derived that the technique of SFE positively affected the exine layer, shown in the observations by Scanning Electron Microscopy (SEM).

中文翻译：

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从安第斯高地森林蜂花粉中超临界 CO2 提取类胡萝卜素

采用超临界流体技术（SFE），以CO2为溶剂，压力28MPa，温度60℃，气流5L/min，时间6h，获得油性蜂花粉提取物。最初，从哥伦比亚的不同城市采集花粉样品，根据总类胡萝卜素（CCT）含量和提取过程的产量进行选择和分析。结果显示，CCT 介于 119 至 2140 µg β-胡萝卜素/克样品之间，获得油状提取物的产率百分比介于 3 – 5% 之间。对于油状提取物中的 CCT，获得了 385–6942 µg β-胡萝卜素/g 提取物，其中 Guatavita 花粉的含量最高。根据调查，作者得出结论，SFE 技术对外壁层产生了积极影响，这在扫描电子显微镜 (SEM) 的观察结果中得到了体现。